Heine studied classical languages and theology before turning to medicine, a decision influenced by his uncle, Johann Georg Heine, who owned a orthopaedic institute in Würzburg. He was awarded a doctorate in 1827. In the 1830s, Jakob Heine opened an orthopaedic institution in Cannstatt and served as director there until 1865.
An honorary citizen of Cannstatt, Heine received the titles of Court counsellor and Privy counsellor, and was raised to the nobility with the Württembergian Order of the Crown.