It can be used to narrow down an investigation amongst a group of criminals with differing MOs. For example, a burgular may always use a back window, broken with a rock. If a group of known criminals are suspected to have commited a burgulary, then the characteristic modus operandi can be used to identify the burgular.
A criminal's MO can also be used in profiling, where the MO can give vital clues to a criminal's psychology.