In the resulting spectra, gamma-ray intensity is plotted as a function of the source velocity. At velocities corresponding to the resonant energy levels of the sample, some of the gamma-rays are absorbed, resulting in a drop in the measured intensity and a corresponding dip in the spectrum. The number, positions, and intensities of the dips (also called peaks) provide information about the chemical environment of the absorbing nuclei and can be used to characterize the sample.
In order to for Mössbauer absorption of gamma-rays to occur, the gamma-ray must be of the appropriate energy for the nuclear transitions of the atoms being probed. Also, the gamma-ray energy should be relatively low, otherwise the system will have a low recoil-free fraction (see Mossbauer effect) resulting in a poor signal-to-noise ratio. Only a handful of elemental isotopes exist for which these criteria are met, so Mössbauer spectroscopy can only be applied to a relatively small group of atoms including: 57Co, 57Fe, 129I, 119Sn, 121Sb. Of these 57Fe is by far the most common element studied using the technique.