Twin studies have been called into question because of the difficulty of isolating environmental factors from genetic factors. For example, twins that are "raised apart" are still often raised in similar environments, even by different parts of the same family. Inadequately controlling for such similarities may bias studies towards overestimating the influence of genetic factors.
Also, there are different ways of calculating concordance (the presence of a similar disease phenotype in twins) which can give markedly different results.
For a group of twins in which at least one member of each pair is affected, pairwise concordance is a measure of how many of each pair will have both members affected. It can be calcuated with a formula of C/C+D, in which C is the number of concordant pairs and D is the number of discordant pairs.
For example, a group of 10 twins have been pre-selected to have one affected member. During the course of the study four other previously non-affected members become affected. This gives a pairwise concordance of 4/(4+6) or 4/10 or 40%.
For a group of twins in which at least one member of each pair is affected, probandwise concordance is a measure of the proportion of twins who have the illness who have an affected twin and can be calculated with the formula of 2C/(2C+D), in which C is the number of concordant pairs and D is the number of discordant pairs.
For example, a group of 10 twins that have been pre-selected to have one affected member. During the course of the study four other previously non-affected members become affected. This gives a probandwise concordance of 8/(8+6) or 8 / 14 or 57%.
Pairwise concordance
Probandwise concordance
Further Reading